Regulatory
NemR (RP)

Part:BBa_K1398008

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-12)

NemR Recognition Promoter

This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism. It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.

An iLOV reporter gene was added upstream of the promoter. The resulting construct is characterised here:

https://parts.igem.org/Part:BBa_K1398007

The full article can be found here:

http://2014.igem.org/Team:Exeter/Detection


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None